USDA-ARS Exotic Pathogens of Citrus Collection
In Beltsville, Maryland, the Exotic Pathogens of Citrus collection (EPCC) maintains an extensive and unique collection of graft transmissible pathogens of citrus. Citrus pathogen collection includes Candidatus Liberibacter species, Xanthomonas strains, Citrus tristeza virus (CTV), Citrus yellow mosaic virus, citrus chlorotic dwarf virus and other non-cultured pathogens.
These have been collected from all over the world and include representatives from the United States.
Materials in the collection is used to provide positive controls upon request to members of the NCPN. These are in the form of RNA, DNA or plant extracts for use in diagnostic tests. Freeze dried plant extracts of infected plants as well as living plants with each of the pathogens are provided.
Diagnostic tests that can be used by the NCPN to provide clean plant materials to customers are developed. A recent example of this is the immune tissue print assay developed for the sensitive and specific detection of ‘Ca. Liberibacter asiaticus’. This assay is currently being evaluated jointly with the National Germplasm Collection at UC Riverside.