NCPN Citrus
NCPN Citrus
NCPN Citrus
University of California
NCPN Citrus

USDA-ARS National Clonal Germplasm Repository For Citrus & Dates (NCGRCD)

 

The mission of the USDA-ARS National Clonal Germplasm Repository For Citrus and Dates (NCGRCD) is to acquire, evaluate, preserve, maintain, and distribute germplasm of Citrus, citrus wild relatives, and date palms mainly for research purposes and to conserve genetic diversity.

 

USDA-ARS NCGRCD protective structures: 4-compartment greenhouse on left and two-compartmen
USDA-ARS NCGRCD protective structures: 4-compartment greenhouse on left and two-compartmen

Once therapeutics and diagnostics are conducted, germplasm is maintained in duplicate within protective structures. Germplasm in the form of budwood (pathogen-tested), fruit, seeds, and pollen is distributed to researchers and certification programs worldwide. 

lab1
 
Lab 2
 
lab 3
Buds are sterilized, excised, and processed to remove all water from plant cells. For each accession, 175 buds are processed and preserved in liquid nitrogen. When removed from storage in liquid nitrogen, plants are regenerated via tissue culture.

 

To secure the genetic diversity of Citrus and citrus relatives for the long term, accessions are either processed at the NCGRCD or sent to the USDA-ARS National Laboratory for Genetic Resources Preservation (NLGRP) in Fort Collins, CO for cryoprocessing. Once preserved in liquid nitrogen, accessions are stored in large cryo-tanks at the NLGRP.

Germplasm can be stored in liquid nitrogen for long term, secure preservation. NCGRCD technician opening a cryo-storage tank.
Germplasm can be stored in liquid nitrogen for long term, secure preservation. NCGRCD technician opening a cryo-storage tank.
 
Large capacity cryo-tanks at the NLGRP in Fort Collins, CO.
Large capacity cryo-tanks at the NLGRP in Fort Collins, CO.
Webmaster Email: jsjacobs@ucdavis.edu